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thruplex tag seq flex library preparation kit  (TaKaRa)


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    Structured Review

    TaKaRa thruplex tag seq flex library preparation kit
    Thruplex Tag Seq Flex Library Preparation Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/thruplex+tag+seq+kit/ThruPLEX+Tag-Seq+HV+Core+Components/bio_rxiv__64898__2026__04__02__715020-298-13-19
    Average 94 stars, based on 1 article reviews
    thruplex tag seq flex library preparation kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Generated:

    Article Title: Essential function of the integrator complex in Kaposi’s sarcoma-associated herpesvirus lytic replication
    Article Snippet: .. The libraries were generated using the ThruPLEX Tag-seq Kit following the manufacturer’s protocol (Takara #R400584). ..

    Amplification:

    Article Title: A large‐scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression‐free survival
    Article Snippet: The coverage and performance of primers were analysed using a 2200 TapeStation instrument (Agilent, Santa Clara, CA, USA) and Hi‐seq 4000. .. The primers were grouped together as 7–8plex, and primers in each group were chosen to target different genes to minimise nonspecific amplification and cross‐reactivity [ ]. sWGS libraries were prepared from 5 ng of cfDNA from each plasma sample or 50 ng of DNA from buffy coat using the ThruPLEX® Tag‐seq Kit (Takara Bio, Inc., Shiga, Japan) as described in the manufacturer's instructions. .. After barcoding PCR, all samples were analysed using 2200 TapeStation (Agilent) to measure the concentration and size of the product.

    Article Title: A large-scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression-free survival.
    Article Snippet: The coverage and performance of primers were analysed using a 2200 TapeStation instrument (Agilent, Santa Clara, CA, USA) and Hi-seq 4000. .. The primers were grouped together as 7– 8plex, and primers in each group were chosen to target different genes to minimise nonspecific amplification and cross-reactivity [17]. sWGS libraries were prepared from 5 ng of cfDNA from each plasma sample or 50 ng of DNA from buffy coat using the ThruPLEX® Tag-seq Kit (Takara Bio, Inc., Shiga, Japan) as described in the manufacturer’s instructions. .. After barcoding PCR, all samples were analysed using 2200 TapeStation (Agilent) to measure the concentration and size of the product.

    Clinical Proteomics:

    Article Title: A large‐scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression‐free survival
    Article Snippet: The coverage and performance of primers were analysed using a 2200 TapeStation instrument (Agilent, Santa Clara, CA, USA) and Hi‐seq 4000. .. The primers were grouped together as 7–8plex, and primers in each group were chosen to target different genes to minimise nonspecific amplification and cross‐reactivity [ ]. sWGS libraries were prepared from 5 ng of cfDNA from each plasma sample or 50 ng of DNA from buffy coat using the ThruPLEX® Tag‐seq Kit (Takara Bio, Inc., Shiga, Japan) as described in the manufacturer's instructions. .. After barcoding PCR, all samples were analysed using 2200 TapeStation (Agilent) to measure the concentration and size of the product.

    Article Title: Genomics and Epigenomics Approaches for the Quantification of Circulating Tumor DNA in Liquid Biopsy: Relevance of a Multimodal Strategy
    Article Snippet: .. Other sWGS approaches for analyzing tumor-associated copy number changes include Plasma-Seq [ ] and the ThruPLEX ® Tag-Seq Kit (Takara, Shiga, Japan) [ ]. ..

    Article Title: A large-scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression-free survival.
    Article Snippet: The coverage and performance of primers were analysed using a 2200 TapeStation instrument (Agilent, Santa Clara, CA, USA) and Hi-seq 4000. .. The primers were grouped together as 7– 8plex, and primers in each group were chosen to target different genes to minimise nonspecific amplification and cross-reactivity [17]. sWGS libraries were prepared from 5 ng of cfDNA from each plasma sample or 50 ng of DNA from buffy coat using the ThruPLEX® Tag-seq Kit (Takara Bio, Inc., Shiga, Japan) as described in the manufacturer’s instructions. .. After barcoding PCR, all samples were analysed using 2200 TapeStation (Agilent) to measure the concentration and size of the product.

    Sequencing:

    Article Title: A large‐scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression‐free survival
    Article Snippet: .. Sequencing libraries for sWGS were prepared using 5 ng of cfDNA from each sample and 50 ng of DNA from buffy coat using the ThruPLEX® Tag‐seq Kit (Takara Bio, Inc., Shiga, Japan) as described in the manufacturer's instructions. ..

    Article Title: Retinoic acid receptor-related orphan receptor α regulates bystander activation of memory CD8 + T cells
    Article Snippet: Chromatin digestion and immunoprecipitation were performed using the SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling) with anti-AM-tag antibody (Active Motif). .. Sequencing libraries were prepared from the ChIP and Input DNAs using the SMARTer ThruPLEX Tag-seq Kit (Clontech) according to the manufacturer’s protocol. .. Single-read sequencing (150 bp) was performed on the DNBSEQ-G400RS sequencer (MGI Tech).

    Article Title: A large-scale retrospective study in metastatic breast cancer patients using circulating tumour DNA and machine learning to predict treatment outcome and progression-free survival.
    Article Snippet: .. Sequencing libraries for sWGS were prepared using 5 ng of cfDNA from each sample and 50 ng of DNA from buffy coat using the ThruPLEX® Tag-seq Kit (Takara Bio, Inc., Shiga, Japan) as described in the manufacturer’s instructions. ..

    Article Title: Improved Quantification of Circulating Tumor DNA in Translocation-Associated Myxoid Liposarcoma by Simultaneous Detection of Breakpoints and Single Nucleotide Variants.
    Article Snippet: Fragment length was assessed using the Fragment Analyzer system (Agilent, Santa Clara, CA, USA). .. For UMI- tagged libraries prepared with the SMARTer ThruPLEX Tag- seq Kit, the Curio Genomics web platform was specifically recommended by the manufacturer as a bioinformatical tool for downstream sequencing analysis. .. First, the sequence reads from cfDNA samples were uploaded to the Curio Genomics web platform (www. curio genom ics. com).

    Chromatin Immunoprecipitation:

    Article Title: Retinoic acid receptor-related orphan receptor α regulates bystander activation of memory CD8 + T cells
    Article Snippet: Chromatin digestion and immunoprecipitation were performed using the SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling) with anti-AM-tag antibody (Active Motif). .. Sequencing libraries were prepared from the ChIP and Input DNAs using the SMARTer ThruPLEX Tag-seq Kit (Clontech) according to the manufacturer’s protocol. .. Single-read sequencing (150 bp) was performed on the DNBSEQ-G400RS sequencer (MGI Tech).



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